Antihyperuricemia of Lemongrass Extract (Cymbopogon citratus) on Male Mice with Potassium Oxonate and Chicken Liver Juice Induced
Main Article Content
Rizky Fadhila
Hotlina Nainggolan
Background: Hyperuricemia is a pathological condition characterized by increased blood uric acid levels exceeding 7 mg/dL. The use of medicinal plants for managing hyperuricemia has garnered significant attention.
Aims & Methods: This study aims to evaluate the anti-hyperuricemic effect of lemongrass extract (Cymbopogon citratus) in a hyperuricemia model using 18 Deutsche Denken Yoken (DDY) mice induced with potassium oxonate and chicken liver juice. Lemongrass simplicia was extracted by maceration using 96% ethanol. Test animals were induced with hyperuricemia by administering chicken liver juice at a dose of 0.5 mL/20 g body weight (BW) for seven consecutive days and potassium oxonate at a dose of 250 mg/kg BW on the eighth day. The test animals were divided into six test groups, namely group 1 was the normal; group 2 was the negative control; group 3 was the positive control (allopurinol at 13 mg/kg BW); groups 4 – 6 were treatment lemongrass extract at doses of 350, 700, and 910 mg/kg BW, respectively.
Result: The results showed that group 6 mice statistically significantly reduced uric acid levels (p = 0.001, p < 0.05). Cymbopogon citratus extract effectively reduces uric acid levels and holds substantial potential as a uric acid-lowering agent, supporting its empirical use in managing hyperuricemia.
Adi, B., Agus, M. I., & Boima, S. (2017). Analisis dan Identifikasi Senyawa Saponin dari Daun Bidara (Zhizipus mauritania L.). Jurnal ITEKIMA, 2(1), 84–94.
Aduwamai, U. H. (2019). Effect of Some Weaning Foods on Weanling Albino Rats. Biomedical Journal of Scientific & Technical Research, 21(2), 15702–15706. https://doi.org/10.26717/bjstr.2019.21.003570
Afiah, N., Santi, I., & Putra, B. (2023). Dose Optimization of Antihyperuricemia Effects of Matoa Leaf (Pometia pinnata J.R.Forst & G.Forst) in Rats. Pharmaceutical Reports, 2(2), 10–13. https://doi.org/10.33096/pharmrep.v2i2.271
Agusfina, M., & Julio, K. (2022). Uji Dan Penetuan Efek Ekstrak Putik Sawo (Manilkara zapota L.) Terhadap Penyembuhan Luka Sayat Pada Mencit Putih Jantan. Journal Pharma Saintika, 6(1), 16–24. https://doi.org/10.51225/jps.v6i1.17
Alfiyanti, W. I., Ferdyanti, A. P., Rohman, A. F., Agustina, E., Purnamasari, R., & Kusumawati, E. (2023). Phtochemical Compounds Test of Lemongrass Extract ( Cymbopogon citratus ) Using Maceration Method. The 3rd International Conference on Sustainable Health Promotion (ICOSHPRO), August, 30–31.
Arifin, W. N., & Zahiruddin, W. M. (2017). Sample size calculation in animal studies using resource equation approach. Malaysian Journal of Medical Sciences, 24(5), 101–105. https://doi.org/10.21315/mjms2017.24.5.11
Badriyah, L., & Farihah, D. A. (2022). Analisis ekstraksi kulit bawang merah ( Allium cepa L . ) menggunakan metode maserasi. 3(1), 30–37.
Bardin, T., Magnat, E., Clerson, P., Richette, P., & Rouchon, B. (2022). Epidemiology of gout and hyperuricemia in New Caledonia. Joint Bone Spine, 89(2), 105286. https://doi.org/10.1016/j.jbspin.2021.105286
BPOM RI. (2023). Berita Negara. Peraturan Badan Pengawas Obat Dan Makanan Nomor 20 Tahun 2023 Tentang Pedoman Uji Farmakodinamik Praklinik Obat Tradisional.
Dalbeth, N., Gosling, A. L., Gaffo, A., & Abhishek, A. (2021). Gout. The Lancet, 397(10287), 1843–1855. https://doi.org/10.1016/S0140-6736(21)00569-9
Dewatisari, W. F. (2020). Perbandingan Pelarut Kloroform dan Etanol terhadap Rendemen Ekstrak Daun Lidah Mertua (Sansevieria trifasciataPrain.)Menggunakan Metode Maserasi. Prosiding Seminar Nasional Biologi Di Era Pandemi COVID-19, September, 127–132.
Djahi, S. N. N. S., Lidia, K., Pakan, P. D., & Amat, A. L. S. (2021). Uji Efek Antidiabetes Ekstrak Etanol Daun Sereh (Cymbopogon citratus) Terhadap Penurunan Glukosa Darah Tikus Putih Sprague Dawley Diinduksi Aloksan. Cendana Medical Journal (CMJ), 9(2). https://doi.org/10.35508/cmj.v9i2.5981
Djoko, W., Taurhesia, S., Djamil, R., & Simanjuntak, P. dkk. (2020). Standardisasi Ekstrak Etanol Herba Pegagan (Centella asiatica). Sainstech Farma, 13(2), 118–123.
Dong, Y., Huang, H., Zhao, M., Sun-Waterhouse, D., Lin, L., & Xiao, C. (2016). Mechanisms underlying the xanthine oxidase inhibitory effects of dietary flavonoids galangin and pinobanksin. Journal of Functional Foods, 24, 26–36. https://doi.org/10.1016/j.jff.2016.03.021
Dwi, Noviyanti. L. Aini. T. Yurika. (2023). Pengaruh Pemberian Kompres Hangat Air Rebusan Serai (Cymbopogon citratus) Terhadap Penurunan Nyeri Arthritis Gout Di Puskesmas Merdeka Palembang. 5, 633–646.
Ema, D., Firstca Aulia, R., Tunik, S., & Ovikariani. (2022). Penetapan Parameter Spesifik Dan Non Spesifik Ekstrak Etanol Biji Buah Trembesi (samanea saman). Benzena Pharmaceutical Scientific Journal, 20(20).
Fardin, & Onsi, R. (2019). The Effect Of Tablets On Alopurinol And Probenecid Tablets On Blood Levels Of Potassium-Induced Blood-Oxonic Acid. Majalah Farmasi Nasional, 16(01), 48–55.
Ferani, C., Siti, M., & Evi, U. U. (2014). Uji Aktivitas Antihiperurisemia Ekstrak n-Heksana, Etil Asetat, dan Etanol 70% Daun Tempuyung (Sonchus arvensis L.) pada Mencit Jantan Hiperurisemia. E-Jurnal Pustaka Kesehatan, 2(2), 205–210.
Fitrya, & Muharni. (2014). Ekstrak Etanol Akar Tumbuhan Tunjuk Langit (Helminthostachys zaylanica Linn Hook) Terhadap Mencit Jantan Galur Swiss. Traditional Medicine Journal, 19(1), 2014.
Halimu, R. B., S.Sulistijowati, R., & Mile, L. (2020). Identifikasi kandungan tanin pada Sonneratia alba. Jurnal Ilmiah Perikanan Dan Kelautan, 5(4), 93–97.
Han, T., Chen, W., Qiu, X., & Wang, W. (2024). Epidemiology of gout - Global burden of disease research from 1990 to 2019 and future trend predictions. Therapeutic Advances in Endocrinology and Metabolism, 15, 20420188241227296. https://doi.org/10.1177/20420188241227295
Haresmita, P. P., & Pradani, M. P. K. (2022). Determination of Total Flavonoid in Jamu “X” With Uv-Visible Spectrophotometric Methods. Jurnal Farmasi Sains Dan Praktis, 8(2), 177–184. https://doi.org/10.31603/pharmacy.v8i2.6864
Hidayati, D. N., Sumiarsih, C., & Mahmudah, U. (2018). Standarisasi Non Spesifik Ekstrak Etanol Daun Dan Kulit Batang Berenuk (Crescentia cujete Linn). Jurnal Ilmiah Cendekia Eksakta, 3(1), 19–23.
Ilkay, O. E., & Fatma, D. S. S. (2021). Natural Products and Extracts as Xantine Oxidase Inhibitors - A Hope for Gout Disease? In Current Pharmaceutical Design (Vol. 27, Issue 2, pp. 143–158). https://doi.org/10.2174/1381612826666200728144605
Kim, C. W., Sung, J. H., Kwon, J. E., Ryu, H. Y., Song, K. S., Lee, J. K., Lee, S. R., & Kang, S. C. (2019). Toxicological evaluation of Saposhnikoviae radix water extract and its antihyperuricemic potential. Toxicological Research, 35(4), 371–387. https://doi.org/10.5487/TR.2019.35.4.371
Kussoy, V. F. M., Kundre, R., & Wowiling, F. (2019). Kebiasaan Makan Makanan Tinggi Purin Dengan Kadar Asam Urat Di Puskesmas. Jurnal Keperawatan, 7(2), 1–7. https://doi.org/10.35790/jkp.v7i2.27476
Maria, U., Ricky Chandra, K., & Metalia, E. (2020). Standardisasi Parameter Spesifik dan Non Spesifik Ekstrak Etanol Daun Jamblang (Syzygium cumini (L.) Skeels). Jurnal Ilmu Farmasi Dan Farmasi Klinik (JIFFK), 17(2), 35–43.
Maryana, H. D., Nainggolan, H., & Hanifa, N. R. (2024). Effect of Suruhan Leaves ( Peperomia pellucida L . Kunth ) Extract on Triglyceride Blood Level in Diabetic Rats. Jurnal Farmasi Dan Ilmu Kefarmasian Indonesia, 11(1), 53–60. https://doi.org/10.20473/jfiki.v11i12024.53-60
Maulid, D., Bahar, B., Sirajuddin, S., Hadju, V., Citrakesumasari, C., & Masni, M. (2020). Effect of the stems lemongrass (Cymbopogon citratus) in pallumara and pepes anchovy (stolephorus sp.) to uric acid levels of hyperuricemia elderly women. Open Access Macedonian Journal of Medical Sciences, 8(T2), 109–114. https://doi.org/10.3889/oamjms.2020.5203
Nawafila, F., & Muhimmatul, K. (2023). Penetapan Parameter Standarisasi Non Spesifik Ekstrak Batang Sereh (Cymbopogon Citrus). Open Journal Systems, 18(1), 153–160.
Nurul, H., Fenny, H., Gunawan, M., Lestari, & Anggun. (2018). Uji Efektivitas Antihiperurisemia Ekstrak Air Daun Salam ( Syzygium polyanthum Wight .) Terhadap Mencit Jantan ( Mus musculus ) Yang Diinduksi Jus Hati Ayam dan Kalium Oksonat. Jurnal Saintika, 18(1), 24–31.
Ojha, R., Singh, J., Ojha, A., Singh, H., Sharma, S., & Nepali, K. (2017). An updated patent review: Xanthine oxidase inhibitors for the treatment of hyperuricemia and gout (2011-2015). Expert Opinion on Therapeutic Patents, 27(3), 311–345. https://doi.org/10.1080/13543776.2017.1261111
Pelleng, M. Y., Roosmarinto, & Rahayu, M. (2019). Efektivitas Seduhan Herbal Serai (Cymbopogon citratus) Terhadap Kadar Asam Urat Darah Tikus Putih (Rattus norvegicus) Jantan Galur Wistar Yang Diinduksi Streptozotocin (STZ).
Rachmanda Haris, S. (2022). Efektivitas Seduhan Sereh (Cymbopogon citratus) untuk Menurunkan Kadar Asam Urat pada Mencit Putih Jantan (Mus musculus). In Politeknik Harapan Bersama Tegal.
Rahayu, M. T., Afifah, A., & Muflikhah, K. (2022). Efek Pemberian Ekstrak Etanol Seledri (Apium graveolens l.) Terhadap Kadar Asam Urat pada Tikus Putih (sprague dawley) Model Chronic Kidney Disease. Mandala Of Health, 15(1), 30. https://doi.org/10.20884/1.mandala.2022.15.1.5784
Rahman, A. O., Herlambang, Simanjuntak, C. A., Dewi, H., Syauqy, A., & Lipinwati. (2019). The High Dose Toxicity Of Betel Nut (Areca catechu L.) on Reproduction Organ of Rats. Jambi Medical Journal : Jurnal Kedokteran Dan Kesehatan, 7(1), 41–49. https://doi.org/10.22437/jmj.v7i1.7059
Rodhiyah, R., Rahmatulloh, A., & Firdaus, R. C. (2024). Perbandingan Analisis Parameter Moisture Content Flavour Powder Menggunakan Moisture Analyzer Dan Oven. DISTILAT: Jurnal Teknologi Separasi, 10(1), 287–295. https://doi.org/10.33795/distilat.v10i1.4877
Rusmini, R., Hesti, K., & Anita, W. (2023). Prevalensi Kejadian Penyakit Tidak Menular (PTM). Jurnal Keperawatan Silampari, 6(2), 1032–1039.
Saharuddin, M., & Titawanno, J. E. (2020). Uji Efektivitas Ekstrak Serai Dapur (Cymbopogon citratus (DC.) Stapf) Terhadap Pertumbuhan Candida albicans Metode. Journal Pharmacy and Sciences, 11(2), 60–69.
Saivarshine, S., Kavitha, S., Vishnupriya, V., & Gayathri, R. (2020). In vitro xanthine oxidase inhibitory potential of flaxseed oil. Plant Cell Biotechnology and Molecular Biology, 21(29–30), 92–96.
Silpiyani, S., Kurniawan, W. E., & Wibowo, T. H. (2023). Karakteristik Responden Lansia Penderita Asam Urat Di Desa Pageraji Kecamatan Cilongok. SENTRI: Jurnal Riset Ilmiah, 2(5), 1818–1828. https://doi.org/10.55681/sentri.v2i5.916
Simorangkir, D. (2020). Uji Efektivitas Antidepresan Ekstrak Batang Serai (Cymbopogon nardus (L.) Rendle) Terhadap Mencit Putih Jantan (Mus musculus). Jurnal Penelitian Farmasi & Herbal, 3(1), 100–105. https://doi.org/10.36656/jpfh.v3i1.331
Sinata, N., Dona, R., & Muthui’ah, M. (2022). Aktivitas antihiperurisemia ekstrak etanol daun pandan wangi (pandanus amaryllifolius roxb.) pada mencit jantan hiperurisemi. Jurnal Ilmiah Farmako Bahari, 13(2), 164. https://doi.org/10.52434/jfb.v13i2.1223
Stamp, L. K., & Chapman, P. T. (2020). Allopurinol hypersensitivity : Pathogenesis and prevention. Best Practice & Research Clinical Rheumatology. https://doi.org/10.1016/j.berh.2020.101501
Sulistiyowati, R., & Kusumaningrum, A. (2023). Pengaruh Air Rebusan Daun Sirsak (Annona muricata Linn) Terhadap Kadar Asam Urat Pada Wanita Usia Lebih Dari 45 Tahun Di Desa Besuki Kecamatan Lumbir Kabupaten Banyumas. The Journal of Muhammadiyah Medical Laboratory Technologist, 6(52), 58. https://doi.org/10.30651/jmlt.v6i1.11932
Sulistyarini, I., Sari, D. A., & Wicaksono, T. A. (2019). Skrining Fitokimia Senyawa Metabolit Sekunder Batang Buah Naga (Hylocereus polyrhizus). Jurnal Ilmiah Cendekia Eksakta, 56–62.
Syifa, N., Nastiti, K., & Darsono, P. V. (2022). Penetapan Kadar Flavonoid Total Pada Tingkatan Fraksi Ekstrak Kulit Pohon Jambu Mete (Anacardium occidentale Linn) Dengan Metode Spektrofotometri Uv-Vis. 1(2), 96–103.
Tambunan, P. M. (2022). Karakterisasi Dan Uji Toksisitas Akut Ekstrak Etanol Daun Serai (Cymbopogon citratus) Desa Bandar Khalipah Terhadap Mencit Jantan (Mus musculus). Jurnal Kimia Saintek Dan Pendidikan, 6(1), 1–10. https://doi.org/10.51544/kimia.v6i1.2968
Tang, D., Ye, Y., Wang, C., & Li, Z. (2017). Potassium oxonate induces acute hyperuricemia in the tree shrew ( tupaia belangeri chinensis ). 66(935), 209–216.
Tri, Y., Reubun, A., Pangalila, A. A., & Oktaviani, L. (2024). Uji Parameter Spesifik dan Non Spesifik Ekstrak Benalu Teh ( Scurrula oortiana Dans .). Jurnal Ilmiah Farmasi Vol, 13(1), 1–6.
Utami, N. R., Rahayuningsih, M., Abdullah, M., & Haka, F. H. (2019). Etnobotani Tanaman Obat Masyarakat Sekitar di Gunung Unggaran Jawa Tengah. Pros Semi Nas Biodiv Indon, 5(2), 205–208. https://doi.org/10.13057/psnmbi/m050210
Wahid, A. R., & Safwan, S. (2020). Skrining Fitokimia Senyawa Metabolit Sekunder Terhadap Ekstrak Tanaman Ranting Patah Tulang (Euphorbia tirucalli L.). Lumbung Farmasi: Jurnal Ilmu Kefarmasian, 1(1), 24. https://doi.org/10.31764/lf.v1i1.1208
Wendersteyt, N. V., Wewengkang, D. S., Abdullah, S. S., & Stout, D. (2021). Antimicrobial Activity Test Of Exstracts And Fractions Of Ascidian Herdmania momus From Bangka Island Waters Likupang Against The Growth Of Staphylococcus aureus, Salmonella typhimurium, and Candida albicans. 10.
Widiyani, T., & Listyawati, S. (2022). Handbook Penggunaan Hewan Laboratorium dalam Uji in Vivo. Nas Media Pustaka.
Widyastiwi, Fajira, N., & Mohammad, R. (2022). Aktivitas Antihiperurisemia Ekstrak Etanol 96% Rimpang Temu Giring (Curcuma heyneana Val.) Pada Mencit Jantan Yang Diinduksi Kalium Oksonat Dan Jus Hati Ayam. Majalah Farmasi Farmakologi Fakultas Farmasi Makassar, 26(2), 52–56. https://doi.org/10.20956/mff.v26i2.20283
Zhang, C., Wang, R., Zhang, G., & Gong, D. (2018). Mechanistic insights into the inhibition of quearcetin on xanthine oxidase. International Journal of Biological Macromolecules, 112, 405–412. https://doi.org/10.1016/j.ijbiomac.2018.01.190
Rizky Fadhila , Gunadarma University, Indonesia
Department of Pharmacy, Gunadarma University, Indonesia
Hotlina Nainggolan , Gunadarma University, Indonesia
Department of Pharmacy, Gunadarma University, Indonesia
Department of Medicine, Gunadarma University, Indonesia
